Role of Agricultural Management in the Provision of Ecosystem Services in Warm Climate Vineyards: Functional Prediction of Genes Involved in Nutrient Cycling and Carbon Sequestration.

(1) Background: Maintaining soil fertility and crop productivity using natural microbial diversity could be a feasible approach for achieving sustainable development in agriculture. In this study, we compared soils from vineyards under organic and conventional management by predicting functional profiles through metagenomic analysis based on the 16S rRNA gene. (2) Methods: The structure, diversity and predictive functions of soil bacteria related to the biogeochemical cycle of the soil were analyzed, including oxidative and hydrolytic C-cycling enzymes, N-cycling enzymes and P-cycling enzymes. The inter-row spontaneous vegetation in the organic vineyards was also characterized. (3) Results: A clear effect of the farming system (organic vs. conventional) and cover management (herbicides plus tillage, mowing only and mowing plus tillage) on bacterial beta diversity and predicted functions was evidenced. While conventional viticulture increased the potential capacity of the soil to regulate the cycling of inorganic forms of N, organic viticulture in general enhanced those functions involving organic N, P and C substrates. Although the soil bacterial community responded differently to contrasting soil management strategies, nutrient cycling and carbon sequestration functions remained preserved, suggesting a high bacterial functional redundancy in the soil in any case. However, most of the predicted bacterial functions related to soil organic matter turnover were enhanced by organic management. (4) Conclusions: We posit the potential for organic viticulture to adequately address climate change adaptation in the context of sustainable agriculture.

Density-Dependent Effects of Simultaneous Root and Floral Herbivory on Plant Fitness and Defense.

Plants are attacked by multiple herbivores, and depend on a precise regulation of responses to cope with a wide range of antagonists. Simultaneous herbivory can occur in different plant compartments, which may pose a serious threat to plant growth and reproduction. In particular, plants often face co-occurring root and floral herbivory, but few studies have focused on such interactions. Here, we investigated in the field the combined density-dependent effects of root-chewing cebrionid beetle larvae and flower-chewing pierid caterpillars on the fitness and defense of a semiarid Brassicaceae herb. We found that the fitness impact of both herbivore groups was independent and density-dependent. Increasing root herbivore density non-significantly reduced plant fitness, while the relationship between increasing floral herbivore density and the reduction they caused in both seed number and seedling emergence was non-linear. The plant defensive response was non-additive with regard to the different densities of root and floral herbivores; high floral herbivore density provoked compensatory investment in reproduction, and this tolerance response was combined with aboveground chemical defense induction when also root herbivore density was high. Plants may thus prioritize specific trait combinations in response to varying combined below- and aboveground herbivore densities to minimize negative impacts on fitness.

Population Dynamics and Tree Damage of the Invasive Chestnut Gall Wasp, Dryocosmus kuriphilus, in Its Southernmost European Distributional Range.

The invasive chestnut gall wasp (CGW), Dryocosmus kuriphilus, the worst pest of chestnut cultivation, has spread worryingly throughout Europe in less than 20 years. Despite the great concern around this pest, little is known about the status in its southernmost distribution in continental Europe. We assessed spatio-temporal patterns in the population dynamics, phenology and tree damage caused by CGW in southern Spain. Likewise, the relationship between these variables and thermal trends was evaluated. We found strong variation in the population dynamics and flight phenology among localities and over time, which were highly influenced by changes in thermal regimes. Specifically, warmer localities and vegetative periods promoted higher population densities, a partial increase in the survival of immature stages, and advanced flight activity. Moreover, tree damage evolved differently over time in each locality, which suggests that local conditions may determine differences in damage evolution. Our findings evidence that great spatio-temporal variability in the CGW populations takes place across invaded areas in its southernmost European distributional range. Although control mechanisms have been introduced, implementation of further control and management measures are critical to cope with this main threat for the chestnut industry and to prevent its spread to nearing chestnut-producing areas.

Pre-dispersal seed predators boost seed production in a short-lived plant.

Pre-dispersal seed predation diminishes fitness and population growth rate of many plant species. Therefore, plants have developed multiple strategies to reduce the harmful effects of this type of herbivory. The present study aims to determine the effect of pre-dispersal seed predators (PSPs) on the fitness of a short-lived herb, and to discern the mechanisms allowing the plants to reduce the impact of pre-dispersal seed predation. Knowing that the interplay between pre-dispersal seed predators and plants is strongly shaped by the presence of other co-occurring organisms, we tested whether detritivores modulate plant responses towards pre-dispersal seed predators. To do so, we experimentally manipulated in the field pre-dispersal seed predators and detritivores interacting with the short-lived herb Moricandia moricandioides. We found that detritivores did not alter the response of plants to PSPs. Strikingly, the plant overcompensated for pre-dispersal seed predation, almost doubling the number of seeds produced. Plant response to PSPs led to substantial changes in shoot architecture, reproductive traits, chemical defences in leaves and seeds and in seed nutrient content. The overcompensating mechanism seems to be meristem activation, which allowed plants to produce more reproductive tissue, and increasing the proportion of ovules that became seeds, a response which specifically compensates for pre-dispersal seed predation. As far as we know, this is the first experimental evidence of a positive effect of PSPs on plant lifetime fitness as a consequence of plant overcompensation.

Transgenerational effects of ungulates and pre-dispersal seed predators on offspring success and resistance to herbivory.

Herbivorous mammals and insect pre-dispersal seed predators are two types of herbivores that, despite their functional and morphological differences, tend to severely impact many plant species, highly decreasing their seed production and even imperiling the performance of their offspring through transgenerational effects. However, how they influence offspring resistance to herbivory remains largely unknown. In this study we experimentally examined the effects of ungulates and pre-dispersal seed predators on seed quality as well as on the emergence, survival and resistance to herbivory of the seedlings of a semiarid herb. We found that ungulates reduced seedling recruitment but increased seedling resistance to leaf miners. These effects were probably a consequence of insufficient carbon provisioning in seeds that reduced seed viability and provoked carbon limitation in seedlings. Pre-dispersal seed predators did not influence seedling recruitment, but seedlings from mothers damaged by ungulates and by pre-dispersal seed predators suffered less herbivory by grasshoppers. Remarkably, intra-individual differences in damage by pre-dispersal seed predators affected the rate of damage underwent by seedlings. That is, seedlings derived from fruits attacked by seed predators were more resistant to herbivores than siblings derived from un-attacked fruits in plant populations exposed to ungulates. To our knowledge, this is the first study reporting variation in transgenerational-induced resistance of seedlings from the same maternal plant. This study is a valuable contribution to the understanding of transgenerational effects of multiple herbivores and their implications for a deeper comprehension of the natural systems in which they co-occur.

Invasive earthworms erode soil biodiversity: A meta-analysis.

Biological invasions pose a serious threat to biodiversity and ecosystem functioning across ecosystems. Invasions by ecosystem engineers, in particular, have been shown to have dramatic effects in recipient ecosystems. For instance, invasion by earthworms, a below-ground invertebrate ecosystem engineer, in previously earthworm-free ecosystems alters the physico-chemical characteristics of the soil. Studies have shown that such alterations in the soil can have far-reaching impacts on soil organisms, which form a major portion of terrestrial biodiversity. Here, we present the first quantitative synthesis of earthworm invasion effects on soil micro-organisms and soil invertebrates based on 430 observations from 30 independent studies. Our meta-analysis shows a significant decline of the diversity and density of soil invertebrates in response to earthworm invasion with anecic and endogeic earthworms causing the strongest effects. Earthworm invasion effects on soil micro-organisms were context-dependent, such as depending on functional group richness of invasive earthworms and soil depth. Microbial biomass and diversity increased in mineral soil layers, with a weak negative effect in organic soil layers, indicating that the mixing of soil layers by earthworms (bioturbation) may homogenize microbial communities across soil layers. Our meta-analysis provides a compelling evidence for negative effects of a common invasive below-ground ecosystem engineer on below-ground biodiversity of recipient ecosystems, which could potentially alter the ecosystem functions and services linked to soil biota.